Effect of Alcohol Caffeine Concentration on Daphnia Magna

Effect of inebriant caffeine Concentration on daphnia MagnaThe Effect of Alcohol and Caffeine Concentrations on daphnia Magna creepThe target of this audition was to study the effects of alcoholic beverage and caffeine on the nervus, using the water flea Magna as the experiment subject. The hypothesis was Increasing the ducking of caffeine in which the water flea Magna is submersed will ca employment its tone arrange to increase, while change magnitude the tightfistedness of alcohol in which it is submerged will cause its tit rate to decrease. To test this hypothesis two daphnia Magna were uncovered to either alcohol or caffeine and its flavor rate recorded by placing it on a microscope slide and figuring the number of heart beats. It was deducted that the alcohol and caffeine had a very notability effect on the heart, with alcohol retarding it down and caffeine stop number it up. At the highest concentration of alcohol, the heart rate slowed and in turn the hig her(prenominal) concentration of caffeine fixed the heart rate by increasing it. I finger that the equal concept of the effect of alcohol and caffeine on Daphnia Magna would be the same concept for its effect on humans also sense alcohol is a depressant it tends to slow down the body as Caffeine is well known for self-aggrandizing our bodies a boost of energy.IntroductionIt was obvious to test the effects that alcohol and caffeine have on the human heart. The effects of alcohol and caffeine were a distinct interest sense they argon drugs that atomic number 18 polish offd on a self-aggrandising scale in society. However, because it is difficult to perform experiments of this genius on humans and get accurate results, we decided to use Daphnia Magna as a substitute for a human. Though humans and Daphnia Magna are different electric organisms, alcohol and caffeine should have an almost same effect on them. The effect when both organisms are undecided to the same amounts of alcohol and caffeine should be more noticeable in Daphnia Magna because they are much smaller, so in turn the chemicals will run for through the Daphnia Magnas form quicker and effect distributively cell more.There are many explanations why Daphnia Magna are commonly used compared to the use of a human as test subject. Firstly, Daphnia Magna are simple organisms compared to humans thus there are less factors that we have no control over what could likely affect the heart rate. Secondly, the heart of the Daphnia Magna is easy to view and examine because of their downright body form. Thirdly, it is logical to use a Daphnia Magna because the effects of the drug will be observed much more cursorily, within moments compared to virtually half an hour in humans. Also, to get usable results, it would be essential to supply a human subject with a reasonable large amounts of alcohol or caffeine, which wouldnt be good for short circumstance health, as well as unscrupulous. The hypothesis under investigation was positive because depressants, like alcohol tend to decrease the activity of the bodys organs, whilst stimulants like caffeine tend to increase such activity.Methods and MaterialsTwo Daphnia Magna specimen were collected from the habitat container and accordingly placed each in their own hanging drop slide carefully so they would not slide all over like they would a traditional insipid slide. This way one slide would have one Daphnia Magna exam the varying concentrations of alcohol and the other Caffeine.In caffeine we readied concentrations of 0.25%, 0.05%, 0.75% and 1.0% levels of concentration. One ingredient held a bottle of distilled water to be used in amongst concentrations. The water allows the Daphnia Magna to rest a moment that way it doesnt die to quickly from the various concentrations. One member will lead astray the experiment by tallying its heart beats per seconds to allow for an average heart rate. The group and so subtracted the wate r from the slide and replaced it with the fist concentration of caffeine counting the heart beat 15 seconds again then quickly taking away the caffeine and replacing it back with distilled water. We repeat this bring and collect the data until all concentrations have be used and the data compiled.Alcohol there were 2%, 4%, 6%, 8%, and 10% of concentrations. Using a Microscope as well as a wise bottle of water to adjust the Daphnia Magna to the stress of the many concentrations makes the experiment a little easier. As we did with the caffeine the group used a fresh Daphnia Magna collected the average heartbeat and began the process of subjecting the Daphnia Magna to the Alcohol concentrations collecting the heart beat data and observing how it changes with each concentration.ResultsResults demonstrates the profound effect the alcohol and caffeine both have on the heart. As shown in figure 1 and 3 the higher the concentration of caffeine the faster the heart becomes. In figures 2 a nd 4 Alcohol seems to have the opposite affect and slows the heart rate down more and more with each concentration. Within our results it can be determined that when humans consume alcohol that they will experience a slowness and often a more depressed feeling. On the flip side when humans are exposed to caffeine it can be seen that there is a develop of energy Caffeine is a important nervous system stimulant. Your central nervous system includes your brain, spinal cord, and the other nerves in your body. Caffeines main effect on your body is to make you feel more awake and alert for a while (FDA, 2007)Table 1 group data for Caffeine concentrationsCaffeine Concentrations and how they affect the heart rateConcentrationHeart say 15 secHeart stride (BPM)0%532160.25%421680.50%391560.75%281121.0%31124 pulp 1 the graph that shows the group average heart rates when exposed to the different concentrations of caffeine provided that the Daphnia Magna heart rate was counted for 15 seconds then calculated to BPMTable 2 Group data for alcohol concentrationsAlcohol concentrations and how they affect the heart rateConcentrationHeart Rate 15 secHeart Rate (BPM)0%31133.332%271084%24966%12688%197610%1456 cipher 2 the graph that shows the group average heart rates when exposed to the different concentrations of alcohol provided that the Daphnia Magna heart rate was counted for 15 seconds then calculated to BPM.Table 3 Class data for caffeine concentrations body of water Control AVG0.25% Caf0.5% Caf0.75% Caf1.0% CafKR216168156112124g145.356849688G2248448452480508 jeep132208164220188G5133.3120112124136Average154.92200193.6206.4208.8Figure 3 the graph that shows the sept average heart rates when exposed to the different concentrations of caffeine. Table 4 Class data for Alcohol concentrationsInitialsWater Control AVG2% Alc4% Alc6% Alc8% Alc10% AlcKR133.3310896687656g154248285236188172G2205.33122401806448Jeep181148152124124108G5205.3240196232212 snowAverage155.8211.2193.816813 2.896.8Figure 4 the graph that shows the class average heart rates when exposed to the different concentrations of alcohol.DiscussionAlcohol affects every organ in the drinkers body and can damage a developing fetus. drink can impair brain function and motor skills heavy use can increase risk of certain cancers, stroke, and liver disease. (Abuse, 2014)